OBJECT To study T.S. of blastula through permanent slide.

                              EXPERIMENT 04

OBJECT

To study T.S. of blastula through permanent slide.

REQUIREMENTS

Permanent slide of blastula, microscope.

PROCEDURE

Fix the slide of T.S. of blastula under microscope. First observe the slide under low power and then under high power of the microscope.

OBSERVATIONS

1. It is a spherical mass of about sixty

four cells.

2. It is composed of an outer envelope of

cells, the trophoblast or trophoecto-derm and inner cell mass (= embryo-blast).

3. Within the envelope there is a fluid

filled cavity called blastocoel.

4.The side of the blastocyst to which the

inner cell mass is attached is called

the embryonic or animal pole, while

the opposite side is the abembryonic

pole.

5.The inner cell mass is the precursor

of the embryo.

PRECAUTIONS

1.First focus the slide under low power and then under the high power of the microscope.

2.Use fine adjustment while focussing the slide under high power of the microscope.